Date of Graduation

Winter 12-16-2025

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Natural Sciences

Thesis Chair

Megan Wise de Valdez

Abstract

Blackflies (Diptera: Simuliidae) serve as vectors for various pathogens of significance, yet their gut microbiomes remain understudied. We characterized the gut microbiome of Simulium mediovittatum in San Antonio, Texas across developmental stages and seasons by collecting larvae and adults over one year at the effluent-fed Medio Creek and found that the gut microbiome shifted between developmental stages but did not change across seasons. The dominant phylum, Proteobacteria, made up 70% of the adult gut microbiomes. We also compared the physicochemical parameters of the effluent-fed larval habitat to a spring-fed habitat to evaluate water quality. We found that aromatic and non-aromatic heteroatom proportions were statistically different, though there was no overall difference across water parameters and inorganic compounds or organic classes. This study contributes to our understanding of the role that development and season play in the gut microbiome of blackflies with implications for pathogen and population management strategies.

Available for download on Wednesday, May 26, 2027

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