Date of Graduation

Spring 5-20-2025

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biology

Thesis Chair

Ashley Teufel

Abstract

Antimicrobial resistance is a major cause of morbidity and mortality in patients with S. aureus infections. In this study, we analyzed genes, molecular mechanisms, and pathways driving drug resistance in S. aureus using network analysis. Using whole-genome sequencing (WGS) data and systems biology approaches, we identified 229 AMR-associated genes and constructed a protein-protein interaction network among these genes. Through network topology and functional enrichment analyses, we not only confirmed their association with resistance, but also highlighted the central roles of these genes in resistance pathways, such as efflux, target replacement, and target protection, which are directly linked to multiple drug classes. This work demonstrates how combining genomics, network analysis, and system biology approaches can aid in identifying specific genes that warrant consideration for future examination. This approach supports broader efforts to mitigate antibiotic resistance by focusing on the most impactful targets, contributing to the design of targeted treatments and improved management strategies.

Available for download on Wednesday, April 01, 2026

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